| ชื่อบทความ | Postharvest biocontrol ability and involved mechanism of volatile organic compounds from Serratia nematodiphila BC-SKRU-1 against Penicillium digitatum tangerine fruit |
|---|---|
| ประเภทการตีพิมพ์ | วารสารวิชาการระดับนานาชาติ |
| ชื่องานประชุมวิชาการ/วารสาร | Food Control |
| ผู้แต่ง |
ไสว บัวแก้ว |
| วันที่ตีพิมพ์/นำเสนอ | 15 ก.ค. 2567 |
| ปีที่ | 2024 |
| ฉบับที่ | 166 |
| หมายเลขหน้า | 1-14 |
| ลักษณะบทความ | |
| Abstract | Volatile organic compounds (VOCs) emitted by microorganisms offer a promising strategy for managing green mold disease in citrus fruits caused by Penicillium digitatum. This study evaluated the biocontrol efficacy of VOCs produced by Serratia nematodiphila BC-SKRU-1 (BC-SKRU-1 VOCs) against green mold in postharvest tangerine fruits, targeting P. digitatum NKP4321. Our findings demonstrated that BC-SKRU-1 VOCs exhibited a broad spectrum of activity against seven plant pathogens, with the highest efficacy observed against NKP4321 (68.21%). A concentration of 106 CFU mL 1 for VOC production by BC-SKRU-1 completely inhibited the mycelial growth of NKP4321, whereas a concentration of 107 CFU mL 1 was required to fully inhibit spore germination. The application of four plates of BC-SKRU-1 VOCs at 108 CFU mL 1 significantly reduced the severity of green mold disease, with optimal biocontrol achieved through continuous fumigation during storage. Notably, 2-nonanol, identified as the predominant component among the 20 VOCs using HS-SPME/GC-MS, exhibited superior antifungal activity both in vitro and in vivo. It effectively decreased ergosterol production and disrupted plasma membrane integrity in NKP4321 cells. Furthermore, 2-nonanol altered the cellular content of non-enzymatic antioxidants (e.g., GSH, GSSG) and enzymatic antioxidants (e.g., CAT, SOD), elucidating its comprehensive antifungal mechanism. These findings highlight the potential of BC-SKRU-1 VOCs, particularly 2-nonanol, as effective biocontrol agents for enhancing postharvest fruit preservation by combating fungal pathogens. |