| ชื่อบทความ | Utilizing Waste from Palm Oil Mills as a Substrate to Produce Antimicrobial Agents Using Fermentation by Streptomyces philanthi RM-1-138: Effectiveness, Stability, and Compound Identification |
|---|---|
| ประเภทการตีพิมพ์ | วารสารวิชาการระดับนานาชาติ |
| ชื่องานประชุมวิชาการ/วารสาร | Waste and Biomass Valorization |
| ผู้แต่ง |
Poonsuk Prasertsan · Teera Chookaew · Benjamas Cheirsilp4 ไสว บัวแก้ว |
| วันที่ตีพิมพ์/นำเสนอ | 8 ส.ค. 2566 |
| ปีที่ | 2023 |
| ฉบับที่ | - |
| หมายเลขหน้า | - |
| ลักษณะบทความ | |
| Abstract | Antimicrobial agents were produced through submerged and solid substrate fermentation using palm oil mill effluent (POME) and oil palm decanter cake (OPDC) as substrates, respectively. This study aimed to compare the effectiveness of extracts from Streptomyces philanthi RM-1-138 that were obtained from POME and OPDC culture against three strains of bacterial plant pathogens and three strains of oil palm pathogenic fungi. The antimicrobial agents produced in the POME medium could inhibit these pathogens better than those produced in the OPDC medium. The antimicrobial agents exhibited strong antimicrobial activity against Xanthomonas axonopodis pv. glycines and X. campestris pv. campestris (MIC 16 μg ml− 1) and Curvularia oryzae and Ganoderma boninense (MIC 128 μg ml− 1). The effect of temperature (− 20 to 121 °C for 15 min) and pH (4.08.0) on the stability of the antimicrobial agents against C. oryzae was investigated. The antimicrobial agents had excellent stability at the highest temperature and a wide range of pH tests. Identification of these antimicrobial agents produced in POME and OPDC substrate using LC-Q-TOF MS/MS revealed 8 and 6 compounds, respectively. These compounds could be functioned as fungicides (oxadixyl), herbicides (chloreturon), insecticides (isoprocarb and xylylcarb), and antibiotics (anisomycin). Therefore, the POME and OPDC mediums has shown promising potential for being utilized as substitute substrates in the production of antimicrobial agents from S. philanthi RM-1-138. |