| ชื่อบทความ | Antifungal and antiaflatoxigenic mechanism activity of freeze-dried culture filtrate of Streptomyces philanthi RL-1-178 on the two aflatoxigenic fungi and identification of its active component |
|---|---|
| ประเภทการตีพิมพ์ | วารสารวิชาการระดับนานาชาติ |
| ชื่องานประชุมวิชาการ/วารสาร | Journal of Applied Microbiology |
| ผู้แต่ง |
Zhiwei Zhang Poonsuk Prasertsan and Yasuhiro Igarashi ไสว บัวแก้ว |
| วันที่ตีพิมพ์/นำเสนอ | 30 ธ.ค. 2565 |
| ปีที่ | 2023 |
| ฉบับที่ | 134 |
| หมายเลขหน้า | 1-9 |
| ลักษณะบทความ | |
| Abstract | Aims: The study reports the antifungal and antiaflatoxigenic mechanism activity of freeze-dried culture filtrate of Streptomyces philanthi RL-1-178 (DCF RL-1-178) against two aflatoxigenic strains (Aspergillus parasiticus and A. flavus) and identification of its active component. Methods and Results: Significant inhibition in ergosterol biosynthesis by the DCF RL-1-178 appeared on the plasma membrane. Moreover, the DCF RL-1-178 showed dose-dependent inhibition of methylglyoxal (MG) (an aflatoxin inducer) biosynthesis and exhibited a novel antiaflatoxigenic action mechanism. Significant impairments in enzymatic [superoxide dismutase (SOD) and catalase (CAT)] and nonenzymatic [oxidized and reduced glutathione (GSH) and ratio of oxidized and reduced glutathione (GSSG)] anti-oxidative defense molecules were observed in the two aflatoxigenic cells. The active component of the DCF RL-1-178 was identified as natamycin. The natamycin exhibited against A. parasiticus and A. flavus with the minimum inhibitory concentration (MIC) values of 0.5 and 1.0μg ml−1, respectively, while the minimum fungicidal concentration values were the same (4.0μg ml−1). Conclusions: The DCF RL-1-178 containing natamycin exhibited the following effects: (1) inhibition of cellular ergosterol biosynthesis on plasma membrane, (2) reduction inMG(aflatoxin inducer) confirmed novel antiaflatoxigenic mechanism of action, and (3) caused remarkable debasement in antioxidant defense enzymes (SOD and CAT) and nonenzymatic defense molecules (GSH and GSSG) revealing biochemical mechanism of action. Significance and Impact of the Study This is the first report on antifungal and antiaflatoxigenic mechanism activity of Streptomyces philanthi RL-1-178 culture filtrate containing natamycin antibiotic. This new findings will broaden its applications as antifungal medicine and natural preservative in food. |