| ชื่อบทความ | Utilization of palm oil mill effluent as a novel substrate for the production of antifungal compounds by Streptomyces philanthi RM-1-138 and evaluation of its efficacy in suppression of three strains of oil palm pathogen |
|---|---|
| ประเภทการตีพิมพ์ | วารสารวิชาการระดับนานาชาติ |
| ชื่องานประชุมวิชาการ/วารสาร | Journal of Applied Microbiology |
| ผู้แต่ง |
Benjamas Cheirsilp Siriporn Yossan Uraiwan Khunjan and Poonsuk Prasertsan ไสว บัวแก้ว วนิดา เพ็ชร์ลมุล |
| วันที่ตีพิมพ์/นำเสนอ | 13 ต.ค. 2564 |
| ปีที่ | 2022 |
| ฉบับที่ | 132 |
| หมายเลขหน้า | 19902003 |
| ลักษณะบทความ | |
| Abstract | Aims: This study aimed to use palm oil mill effluent (POME) as a renewable resource for the production of antifungal compounds by Streptomyces philanthi RM-1- 138 against Ganoderma boninense, Ceratocystis paradoxa and Curvularia oryzae. Methods and results: The efficacy of antifungal compounds RM-1- 138 against the three strains of fungal oil palm pathogen was evaluated both in vitro and on oil palm leaf segments. In vitro studies using confrontation tests on glucose yeast-malt extract (GYM) agar plates indicated that the strain RM-1- 138 inhibited the growth of all three fungal pathogenic strains. The antifungal compounds produced in the GYM medium exhibited significantly higher inhibition (79%100%) against the three fungal pathogens than using the diluted POME (50%) medium (80%83% inhibition). The optimum condition for the production of antifungal compounds from the strain RM-1- 138 was as following: POME of 47,966 mg L−1 chemical oxygen demand (COD), the initial pH at 7.0 and supplemented with yeast extract (0.4%). Meanwhile, severe morphological and internal abnormalities in C. oryzae hyphae were observed under a scanning electron microscope and transmission electron microscope. In vivo experiment on oil palm leaf segments indicated that the efficacy of the antifungal compounds RM-1- 138 (DSI = 1.3) were not significantly difference in the suppression of Curvularia leaf spot compared with the two commercial chemical fungicides of mancozeb® (DSI = 1.0) and tetraconazole® (DSI = 1.3). Conclusions: Antifungal compounds produced by S. philanthi RM-1- 138 grown in POME have the potential to inhibit fungal pathogens. Significance and impact of the study: The POME (about 47 mg L−1 COD) with the initial pH of 7.0 and supplementation of 0.4% nitrogen could be used as a culture medium for the growth and production of antifungal compounds of S. philanthi RL-1- 138. In addition, the antifungal compound RM-1- 138 could suppress the three strains of oil palm fungal pathogen tested on oil palm leaf segment. |